Research Article | Open Access

Volume 2025 |Article ID 100041 | https://doi.org/10.1016/j.plaphe.2025.100041

Monitoring and Risk Prediction of Low-Temperature Stress in Strawberries through Fusion of Multisource Phenotypic Spatial Variability Features

Nan Jiang,¹ Zaiqiang Yang ,¹ Hanqi Zhang,¹ Chengjing Zhang,¹ Canyue Wang,¹ Na Wang,¹ and Chao Xu²

¹School of Ecology and Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing, 210044, PR China
²Institute of Horticulture, Jiangxi Academy of Agricultural Sciences, Nanchang, 330299, PR China

Abstract

Capturing crop physiological information by phenotyping is a key trend in smart agriculture. However, current studies underutilize spatial structural information in phenotypic imaging. To evaluate the feasibility of crop cold stress monitoring based on phenotypic spatial variability, we conducted controlled experiments on ‘Toyonoka’ strawberry plants under four dynamic cooling gradients and three stress durations and analyzed the dependence of their photosynthetic physiology and phenotypic traits on temperature-time interactions. The results revealed that NPQ/1D-Parallel/TENT, Y(NO)/2D-Region/INEM, and qP/1D-Parallel/TENT presented the highest mutual information, with the maximum net photosynthetic rate (Pmax), relative electrolyte conductivity (REC), and total chlorophyll content (Chla + b), respectively. The difference between the Photosynthetic Physiological Potential Index (PPPI) and relative negative accumulated temperature (RNAT)/650 effectively was used to calculate the cold damage risk (CDRI). An XGBoost-based model integrating the PPPI and RNAT outperformed AdaBoost and RandomForest, achieving an R2 of 0.98, an RMSE of 0.337, a classification accuracy of 92.13 %, and a Kappa coefficient of 0.904. qP/1D-Parallel/TENT contributed the most to the model. This study provides a scientific basis for phenotypic information mining and agro-meteorological disaster monitoring.